Балл 0
Отсутствует экспертное заключение на публикацию
КБПР (методика 2025 г.) = 8.571
Количество соавторов публикации - 7
Публикация в изданиях из Белого списка уровень: 1, K=20
Публикация в изданиях из Белого списка уровень: 1, K=20
-
Рылькова О. А.: количество аффилиаций - 1, вклад в КБПР публикации:
формула: K/(Nсоавторов*Nаффил) = 20/(7*1) = 2.857 -
Гудвилович И. Н.: количество аффилиаций - 1, вклад в КБПР публикации:
формула: K/(Nсоавторов*Nаффил) = 20/(7*1) = 2.857 -
Боровков А. Б.: количество аффилиаций - 1, вклад в КБПР публикации:
формула: K/(Nсоавторов*Nаффил) = 20/(7*1) = 2.857
Статья в периодическом издании
Comparison between newly isolated β-carotene-producing Dunaliella salina and Dunaliella parva strains: genetic and carotenoid characterization
WoS 3.600/Q1 SCOPUS 0.641/Q2 БС 1
| DOI | https://doi.org/10.1007/s10811-026-03939-0 |
|---|---|
| Язык | Английский |
| Журнал | Journal of Applied Phycology ISSN: 0921-8971; Онлайн ISSN: 1573-5176 |
| Год | 2026 |
| Выходные данные | |
| Авторы | |
| Даты |
Поступила в редакцию: 14.04.2026 После доработки: 01.06.2026 Принята к публикации: 05.06.2026 Опубликована онлайн: 19.06.2026 |
| Абстракт | Six carotenogenic strains of halophilic microalga Dunaliella were isolated for the first time from hypersaline environments in Crimea: Lakes Sasyk-Sivash, Sakskoye, Oyburskoye, Moynakskoye, Genicheskoye, and Sivash Bay and characterized through an integrative approach combining morphological, molecular, and biochemical analyses. Unialgal cultures were obtained and examined by light microscopy, scanning electron microscopy, and imaging flow cytometry (FlowCam). Morphometric analysis revealed two distinct size classes: strains from Lakes Sakskoye and Genicheskoye possessed significantly smaller cells than those from the remaining four localities. Discriminant analysis of FlowCam data demonstrated that combining morphometric and chromaticity parameters yielded optimal classification accuracy, substantially exceeding that achieved by either parameter set alone. Phylogenetic reconstruction based on the nuclear ITS region resolved the isolates into two species: Dunaliella salina (Lakes Sasyk-Sivash, Oyburskoye, Moynakskoye, and Sivash Bay) and Dunaliella parva (Lakes Sakskoye and Genicheskoye), revealing substantial genetic diversity among geographically proximate hypersaline habitats. Combined stress treatment (high irradiance and exogenous H2O2) induced carotenoid hyperaccumulation in all strains. Spectrophotometric and HPLC analyses showed that D. salina strains accumulated markedly higher total carotenoid levels, with β-carotene as the dominant pigment, whereas D. parva strains exhibited approximately ten-fold lower carotenoid content, dominated by lutein. Among the D. salina isolates, the Sivash Bay strain displayed the highest carotenoid productivity. These findings establish the Crimean Dunaliella isolates as promising natural sources of β-carotene and lutein for biotechnological applications and highlight the value of polyphasic taxonomy for strain selection. Ключевые слова: Dunaliella salina, Dunaliella parva, Chlorophyceae, β-carotene, Hypersaline environments, Lutein, ITS molecular markers |
| Сведения о финансировании, указанные в публикации | The work was undertaken within the project No. 075–15-2025–605 from the Ministry of Science and Education of Russian Federation. |
| URL | https://link.springer.com/article/10.1007/s10811-026-03939-0 |
| Дополнительные сведения |
ЦКП «Коллекция гидробионтов Мирового океана»:
...The strain from Sivash Bay was isolated in 2001 by FRC IBSS staff, identified on the basis of physiological and morphological traits as D. salina, and subsequently deposited in the «Hydrobionts Collection of the World Ocean» Core Facility of FRC IBSS under the accession acronym IBSS-1.... (стр. 3) СЭМ: ...Scanning electron microscopy (SEM) was performed on a Hitachi SU3500 instrument (Hitachi, Japan) at × 6,000 magnification.... (стр. 4) |
Запись создана: 15-07-2026 19:02
Последнее изменение: 15-07-2026 19:04
Библиографическая ссылка:
Degtyar I., Velyaev Yu., Osokin A., Rylkova O., Gudvilovich I., Borovkov A., Lantushenko A. Comparison between newly isolated β-carotene-producing Dunaliella salina and Dunaliella parva strains: genetic and carotenoid characterization // Journal of Applied Phycology. 2026. https://doi.org/10.1007/s10811-026-03939-0 (Online first)
[WoS 3.600/Q1][SCOPUS 0.641/Q2][БС 1]
Degtyar I., Velyaev Yu., Osokin A., Rylkova O., Gudvilovich I., Borovkov A., Lantushenko A. Comparison between newly isolated β-carotene-producing Dunaliella salina and Dunaliella parva strains: genetic and carotenoid characterization // Journal of Applied Phycology. 2026. https://doi.org/10.1007/s10811-026-03939-0 (Online first)
[WoS 3.600/Q1][SCOPUS 0.641/Q2][БС 1]
Экспертное заключение: –
Белый список
Индексация на момент включения в базу:- Уровень БС
- 1
Web of Science
- Статус
- Да
- Импакт-фактор/Квартиль(год)
- 3.600/Q1 (2025)
- Идентификатор
- –
- Статус
- Да
- Импакт-фактор/Квартиль(год)
- 0.641/Q2 (2025)
- Идентификатор
- –
- Статус
- Нет
- ID
- –
- EDN
- –
Тэги:
ЦКП Коллекция гидробионтов Мирового океана
СЭМ
СЭМ